Measurement of foot thickness and IL-1β level. Soyeom pharmacopuncture reduces foot thickness and IL-1β level in MSU-induced gout mouse. Data are presented as mean ± SD (n = 6 mice per group). *p < 0.05 vs. Control group, ^#p < 0.05 vs. MSU group. MSU, monosodium urate; SY, Soyeom; SBV, sweet bee venom.|@|~(^,^)~|@|Measurement of cytotoxicity and inflammatory cytokines level on BMDMs. BMDMs were treated with different concentration of Soyeom pharmacopuncture (0.32, 0.63, 1.25, 2.5 and 5%). Cytotoxicity of Soyeom pharmacopuncture showed in 5%. LPS primed inflammatory cytokines on BMDMs were treated with different concentration of Soyeom pharmacopuncture (0.63, 1.25, and 2.5%). Data are presented as mean ± SD (3 wells of cell per group). *p < 0.05 vs. Control group. LPS, lipopolysaccharide; SY, Soyeom; IL-6, interleukin 6; TNF-α, tumor necrosis factor alpha.|@|~(^,^)~|@|RNA sequencing analysis. Soyeom pharmacopuncture down regulated genes associated with inflammatory response in MSU-induced gout mouse model.|@|~(^,^)~|@|Analysis of gene expression correlated with inflammatory signal pathway. Soyeom pharmacopuncture showed that down regulated genes associated with IL-1β production and NF-κB pathway signal.

J Pharmacopuncture 2022;25:396~403 https://doi.org/10.3831/KPI.2022.25.4.396